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#Biomolecules Identification
1. A- I would test for a monosaccharide by mixing ml of a particular solution with 6 ml of Benedict's solution in a test tube. I would then heat the test tube in a boiling water for three minutes, then let it cool. If the solution turns a yellow/orange/red color it is positive for glucose. If the solution stays blue then there is no presence of glucose.
1. B- I would test for starch by placing ml of a particular solution in a test tube. I would then add - drops of Iodine. If the Iodine turns blue/black, starch is present.
1. C- I would test lipids by placing a small amount of a solution in a test tube. I would then add several drops of Sudan IV. I would then shake the solution vigorously and set it aside for observation. When the mixture has settled I would observe for the presence of droplets. The solution is positive for lipids if it has formed pink droplets.Help with essay on biomolecules
1. D- I would test for the presence of proteins by placing a particular solution in a test tube. I would then add 10-15 drops of Biuret solution . If the solution turns blue/violet color, proteins are present. I could also perform the same test using Nitric acid. The solution would then turn a yellow color if proteins are present.
. Sucrose is a disaccharide. To do a Benedict solution test I would have to hydrolyze sucrose into its two monosaccarides. To break it down I would put 5 ml of sucrose solution and ml of 1 M Hydrochloric Acid in a test tube. I would then heat the solution in boiling water for minutes. Then I can perform the Benedict solution test.
. Benedicts solution reacts with exposed aldehyde groups.
4. A- To hydrolyze a solution means to break a compound down into monosaccarides.
B- It is down with sucrose because sucrose is a disaccharide. For sucrose to be tested for glucose it has to be broken down into monosaccarides for the test to give a positive result.
5. Biuret reagent reacts with peptide bonds.
6. A- A 5% Vitamin C solution is more concentrated than a 10% Vitamin C solution.
B- I Can prove this by performing the following test
1. I would put 10 ml of Vitamin C indicator into test
tubes.
. I would then add drops of 5%Vitamin C solution in
one test tube and 10% solution in the other.
. Which ever solution turns colorless first has the higher.
concentration of Vitamin C
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